https://novo.goldenmidas.net/story.php?title=kg-i-p-perfused-transcardially-with-chilled-pbs-and-decapitated-the Kg, i.p., perfused transcardially with chilled PBS and decapitated. The lungs were collected and employed fresh or stored in liquid nitrogen for additional use. For protein isolation, one hundred mg wet weight of lung was homogenized on ice using a tissue tearer in 500 l lysis buffer 40 mM Tris pH 7.5, eight M urea, 2.5 M thiourea, 3 33Cholamidopropyl dimethylammonio1propanesulfonate CHAPS, ten Wed, 12 Jul 2023 21:17:23 UTC en